Hepatic and renal iron status in control (+/−) and mutant (<i>ms/−</i>) mice.

<p>Non-haem hepatic (<b>A</b>) and renal (<b>C</b>) iron content was measured as described in the Experimental section. Values are expressed as the means ± S.D. for both liver and kidney samples obtained from 15 mice of each genotype. L-ferritin levels in hepatic (<b>B</b>) and renal (<b>D</b>) cytosolic protein extracts (50 µg/lane) were assessed by western blot analysis <i>left-hand panels</i>. The blots were reprobed with polyclonal anti-human actin antibody as a loading control. (<b>B</b>) and (<b>D</b>) <i>right-hand panels</i> Immunolabelled ferritin bands from four mice were quantified using a Molecular Imager and ferritin protein levels (means ± S.D.) are plotted in arbitrary units. ** – P<0.01.