Genomic organization of the Japanese Crested Ibis MHC class II region.

<p>Three contigs representing different haplotypes were constructed. <i>Collagen-type XI α-2</i> (<i>COL11A2</i>), MHC-IIA (<i>α</i>), MHC-IIB (<i>β</i>) and <i>bromodomain-containing 2</i> (<i>BRD2</i>) genes and their orientations are indicated. Locus names are indicated below the MHC-IIA and -IIB genes. The types of MHC-IIB exon 2 sequences (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108506#pone-0108506-t001" target="_blank">Table 1</a>) are shown below the locus names. B, K, P, S, Sc, and X represent restriction sites used for subcloning of <i>Bam</i>HI, <i>Kpn</i>I, <i>Pst</i>I, <i>Sal</i>I, <i>Sac</i>I, and <i>Xho</i>I, respectively. Solid bars below the map represent locations of isolated lambda phage clones. The bidirectional arrow above <i>DAB2</i> in haplotype 3 indicates the first amplified MHC-IIB fragment.