<p>Denaturing gel electrophoresis (4–12%) and subsequent western blotting of cleared lysates. UT; Untransfected cell lysate, WT; wildtype PDE5A, Ser102A; Ser102A mutant PDE5A, Ser104A; Ser104A mutant PDE5A, Ser102/Ser104A; Ser102A/Ser104A mutant PDE5A.</p
<p>(<b>A</b>) Schematic localization of Ad5 pVII lysine (K) residues in the protein. (<b>B</b>) Muta...
<p>(A) Gel-filtration chromatogram of Gly361Tyr mutant showing partial dissociation of dimers into m...
<p>(A) A fraction of the electrophoregrams corresponding to the sequence of GBF1 from the indicated ...
<p>Native gel electrophoresis (3–12% gel) and subsequent western blotting of cleared lysates. UT; Un...
<p>(A) The cells were observed under an inverted fluorescence microscope (100×). Panel a, mock-trans...
<p>Cell lines modified to contain a single copy of the wild type (CCGAC) or cysteine mutant <i>GPI-P...
<p>(A) Cell lines modified to contain a single copy of the wild type (CCGAC) or cysteine mutant <i>G...
<p>MARC-145 cells were transfected with variants of GP5–HA or with empty plasmid (Ø), subsequently l...
<p><b>A</b> Wild type <i>S</i>. <i>pyogenes</i> D471, sortase mutant AR01, complemented AR01+pAR107,...
<p>SK-N-AS cells were transfected with constructs overexpressing PDE5A-GFP fusion proteins. <b>A</b>...
<p>(A) 293T cells were transfected with various GFP-Tat plasmids or the empty vector, and the fluore...
<p>A) R6-S74A mutant possesses a shorter half-life than wild type protein. Hek293 cells were transfe...
<p>All proteins are shown in the order: untagged (e.g. SOD1<sup>WT</sup>), GFP-tagged at the amino-t...
<p>(A) Structures of L-PRX and its PDZ-deleted mutants (DPDZ-L-PRX). Amino acid residues flanking ea...
<p>Upper panels: twelve single amino acid mutants of p25 were fused with GFP and expressed in epider...
<p>(<b>A</b>) Schematic localization of Ad5 pVII lysine (K) residues in the protein. (<b>B</b>) Muta...
<p>(A) Gel-filtration chromatogram of Gly361Tyr mutant showing partial dissociation of dimers into m...
<p>(A) A fraction of the electrophoregrams corresponding to the sequence of GBF1 from the indicated ...
<p>Native gel electrophoresis (3–12% gel) and subsequent western blotting of cleared lysates. UT; Un...
<p>(A) The cells were observed under an inverted fluorescence microscope (100×). Panel a, mock-trans...
<p>Cell lines modified to contain a single copy of the wild type (CCGAC) or cysteine mutant <i>GPI-P...
<p>(A) Cell lines modified to contain a single copy of the wild type (CCGAC) or cysteine mutant <i>G...
<p>MARC-145 cells were transfected with variants of GP5–HA or with empty plasmid (Ø), subsequently l...
<p><b>A</b> Wild type <i>S</i>. <i>pyogenes</i> D471, sortase mutant AR01, complemented AR01+pAR107,...
<p>SK-N-AS cells were transfected with constructs overexpressing PDE5A-GFP fusion proteins. <b>A</b>...
<p>(A) 293T cells were transfected with various GFP-Tat plasmids or the empty vector, and the fluore...
<p>A) R6-S74A mutant possesses a shorter half-life than wild type protein. Hek293 cells were transfe...
<p>All proteins are shown in the order: untagged (e.g. SOD1<sup>WT</sup>), GFP-tagged at the amino-t...
<p>(A) Structures of L-PRX and its PDZ-deleted mutants (DPDZ-L-PRX). Amino acid residues flanking ea...
<p>Upper panels: twelve single amino acid mutants of p25 were fused with GFP and expressed in epider...
<p>(<b>A</b>) Schematic localization of Ad5 pVII lysine (K) residues in the protein. (<b>B</b>) Muta...
<p>(A) Gel-filtration chromatogram of Gly361Tyr mutant showing partial dissociation of dimers into m...
<p>(A) A fraction of the electrophoregrams corresponding to the sequence of GBF1 from the indicated ...
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