Assessment of hepatic inflammation and steatosis in 12/15-LOKO mice on HFH diet.

<p>The effect of 12/15-lipoxygenase deficiency on (A) terminal body weight, food intake and gonadal white adipose tissue (gWAT) weight expressed as percent total body weight, and hepatic expression of (B) regulators of lymphocyte homing response, (C) pro/anti-inflammatory cytokines and (D) neutrophil chemoattractants. (E) Haematoxylin-eosin and Oil red O staining of paraffin-embedded liver sections to show hepatic steatosis. (F) Quantitative grading scale assessing the quantity of hepatic triglycerides, normalized to signal in WT NC (n = 7) mice (expressed as a fold increase in stained area relative to WT NC mice). (G) Plasma concentrations of oxidized metabolites of arachidonic acid in KO HFH n = 4 (dark gray bars) compared to WT HFH n = 7 (black bars) mice. Values are mean ±SE. *P<0.05, **P<0.01 WT HFH vs. 12/15-LOKO HFH. Scale bar, 50 µm. WT NC SEs for genes Ccr7, Ccl19, Ccl21, Ifng, Tnfa, Il10, Cxcl1 (KC), Cxcl2/3 (MIP-2) were 0.20, 0.21, 0.15, 0.08, 0.12, 0.17, 0.14, 0.13, respectively. WT NC SE value for oil red O stain was 0.15. KO, 12/15 lipoxygenase knockout; HFH, high dietary ω6/ω3 ratio; gWAT, gonadal white adipose tissue; HETE, hydroxyeicosatetraenoic acid.