<p>For each substrate stiffness, nearly 100 cells were imaged for each type of cell. The data from all the substrates for, each type of cell, is pooled together and plotted after binning as done previously for mMSCs. The error bars here represents SE. The correlated behavior between cell and nuclear projected area seem to be roughly intact even across these different cell types although the nuclear projected areas is reaching saturation at different values.</p
Representative 3 plots for each experimental condition are shown. Cells were incubated in the presen...
<p>(a, b, c) Confocal images of a cell on a 70 kPa stiffness substrate. The images show actin filame...
<p>(a–c) Phase contrast images of the harvested HCT-8 R cells, HCT-8 E cells, and normal MKF cells o...
<p>(a) Cell and nuclear projected area as a function of Young's modulus of the substrate. Each point...
<p>(a) Cell area and nuclear projected area as a function of time for cells grown on plastic surface...
<p>Normalized density of each cell phenotype versus substrate stiffness during identical times as a ...
<p>(A) Phase contrast images of cells one day after seeding on FN-gels of increasing rigidity or in ...
(A) Flatness, gray level non-uniformity. (B) Minor axis length, flatness. (C) First order minimum, e...
<p>A) Cell nuclei size differs significantly between cells cultured on different substrates. B) The ...
<p>(<i>A</i>) Visualization of cell shapes and substrate strains in absence of external strain. Line...
<p>Representative SEM micrographs of cells incubated on substrates for 3 days, a) C-HA and b) F-HA a...
<p>A. Montage of images depicting WT (AX3) cells aggregating on the 4 different surfaces, shown at 5...
<p>Projected cell area shows a biphasic relationship with substrate stiffness (A) and a direct relat...
(A) Dissemination of cells from the multicellular spheroids with transient stimulation at 0 hours (l...
Additional file 1: Figure S1. (A) A phase contrast image of TCPS surface. Bar, 100 μm. (B) An images...
Representative 3 plots for each experimental condition are shown. Cells were incubated in the presen...
<p>(a, b, c) Confocal images of a cell on a 70 kPa stiffness substrate. The images show actin filame...
<p>(a–c) Phase contrast images of the harvested HCT-8 R cells, HCT-8 E cells, and normal MKF cells o...
<p>(a) Cell and nuclear projected area as a function of Young's modulus of the substrate. Each point...
<p>(a) Cell area and nuclear projected area as a function of time for cells grown on plastic surface...
<p>Normalized density of each cell phenotype versus substrate stiffness during identical times as a ...
<p>(A) Phase contrast images of cells one day after seeding on FN-gels of increasing rigidity or in ...
(A) Flatness, gray level non-uniformity. (B) Minor axis length, flatness. (C) First order minimum, e...
<p>A) Cell nuclei size differs significantly between cells cultured on different substrates. B) The ...
<p>(<i>A</i>) Visualization of cell shapes and substrate strains in absence of external strain. Line...
<p>Representative SEM micrographs of cells incubated on substrates for 3 days, a) C-HA and b) F-HA a...
<p>A. Montage of images depicting WT (AX3) cells aggregating on the 4 different surfaces, shown at 5...
<p>Projected cell area shows a biphasic relationship with substrate stiffness (A) and a direct relat...
(A) Dissemination of cells from the multicellular spheroids with transient stimulation at 0 hours (l...
Additional file 1: Figure S1. (A) A phase contrast image of TCPS surface. Bar, 100 μm. (B) An images...
Representative 3 plots for each experimental condition are shown. Cells were incubated in the presen...
<p>(a, b, c) Confocal images of a cell on a 70 kPa stiffness substrate. The images show actin filame...
<p>(a–c) Phase contrast images of the harvested HCT-8 R cells, HCT-8 E cells, and normal MKF cells o...