<p>(A) Southern blot analysis. A part of <i>Amp</i><sup>r</sup> gene (0.7-kb) was amplified by PCR and used as the probe. (B) Primers designed for the insertion analysis of the target gene in A2-2 (<i>celA</i>) and D1-B1 (<i>celC</i>), and the theoretical results of PCR using the designed primers.</p
<p>Primer sequences used to obtain the transforming constructs and to identify mutants by PCR and So...
487-494Studies have been able to determine the distribution and diversity of cellulase genes in soil...
Recombinant DNA techniques were used to clone and isolate Cellulo- monas fimi cellulase genes. A se...
<p>PCR was performed with the indicated primer sets in Materials and methods. The type of genome-int...
<p>Primers specific for pRP1195 and chromosomal sequences were used in PCR reactions with chromosoma...
<p>Four sets of Alu-PCR were performed (See <a href="http://www.plosone.org/article/info:doi/10.1371...
<p>A: Genomic DNA of <i>A</i>. <i>colbecki</i> digested with <i>Eco</i> RI and Southern blot hybridi...
Eight genes encoding cellulolytic enzymes were obtained by direct PCR amplification of genomic DNA r...
<p>Total genomic DNA from CHO-K1 clones harbouring stably transfected 1.5kb A2UCOE (3’ HC 5’ LC 5’ H...
<p>1 and 2 indicate two different colonies obtained under each condition tested. Cm40/10 refers to t...
<p>Genomic DNA of <i>A</i>. <i>colbecki</i> digested with <i>Eco</i> RI and Southern hybridization p...
<p>A&B: Southern blot analysis of total DNA using <i>WsCPR1</i> and <i>WsCPR2</i> as a probe. Total ...
<p>Primers used in this study to generate the gene fragments for qRT-PCR analysis (a) and Southern B...
<p>(A) Schematic illustration of the screening procedures for genes that affect Type II survivor for...
PCRs were performed using the DNA isolated from a single egg as a template, followed by a nested (fo...
<p>Primer sequences used to obtain the transforming constructs and to identify mutants by PCR and So...
487-494Studies have been able to determine the distribution and diversity of cellulase genes in soil...
Recombinant DNA techniques were used to clone and isolate Cellulo- monas fimi cellulase genes. A se...
<p>PCR was performed with the indicated primer sets in Materials and methods. The type of genome-int...
<p>Primers specific for pRP1195 and chromosomal sequences were used in PCR reactions with chromosoma...
<p>Four sets of Alu-PCR were performed (See <a href="http://www.plosone.org/article/info:doi/10.1371...
<p>A: Genomic DNA of <i>A</i>. <i>colbecki</i> digested with <i>Eco</i> RI and Southern blot hybridi...
Eight genes encoding cellulolytic enzymes were obtained by direct PCR amplification of genomic DNA r...
<p>Total genomic DNA from CHO-K1 clones harbouring stably transfected 1.5kb A2UCOE (3’ HC 5’ LC 5’ H...
<p>1 and 2 indicate two different colonies obtained under each condition tested. Cm40/10 refers to t...
<p>Genomic DNA of <i>A</i>. <i>colbecki</i> digested with <i>Eco</i> RI and Southern hybridization p...
<p>A&B: Southern blot analysis of total DNA using <i>WsCPR1</i> and <i>WsCPR2</i> as a probe. Total ...
<p>Primers used in this study to generate the gene fragments for qRT-PCR analysis (a) and Southern B...
<p>(A) Schematic illustration of the screening procedures for genes that affect Type II survivor for...
PCRs were performed using the DNA isolated from a single egg as a template, followed by a nested (fo...
<p>Primer sequences used to obtain the transforming constructs and to identify mutants by PCR and So...
487-494Studies have been able to determine the distribution and diversity of cellulase genes in soil...
Recombinant DNA techniques were used to clone and isolate Cellulo- monas fimi cellulase genes. A se...