The ratio of CpG sites in iPSCs was significantly larger than that of the differentiated cells.

<p>(A) Comparisons of 485,577 CpG sites between two groups show high similarities between AF-IPS and FF-IPS cells. (B) In contrast, 12.34% and 12.47% of CpG sites are differentially methylated in AF-IPS and FF-IPS cells, respectively, compared with their parental cells (AF and FF). It should be noted that 89.46% and 86.06% of the differentially methylated sites (DMSs) are hypermethylated in AF-IPS and FF-IPS cells, respectively, compared with their parental cells.