Effects of capsaicin on normal human hepatocytes compared with HepG2 cells.

<p>Cells were treated with 0.5 mM concentration of capsaicin for 3 h or 6 has indicated. The control was defined as cells treated with a medium or vehicles without capsaicin. (<b>A</b>) The bar graph shows the quantification of band intensity as a ratio of FASN protein band intensity relative to the β-actin band intensity in the same blot (e.g. FASN/β-actin). (<b>B</b>) For the effect of capsaicin on intracellular long chain fatty acid and (<b>C</b>) triglyceride synthesis determined by using the free fatty acid and triglyceride quantification kit, data are represented as percentage of the individual value in nmol/µL compared to 100% of control values. (<b>D</b>) The bar chart shows the percentage of disruption of mitochondrial membrane potential relative to the control (set as 100%) after 6 h of capsaicin treatment by JC-1 fluorescence dye staining and detected by flow cytometry. Data from at least three independent experiments performed in triplicate are shown as means ± SD, n = 3, *<i>p</i><0.05.