Effects of C75 on FASN protein expression, free fatty acid levels, triglyceride levels, and disruption of ΔΨm in HepG2 cells.

<p>HepG2 cells were treated with C75 at 0.1 mM concentration for 6 h and 24 h. The control was defined as cells treated with a medium or vehicles without C75. (<b>A</b>) For the effect of capsaicin on the expression of FASN protein level in HepG2 cells, β-actin was used as the internal standard. The bar graph shows the quantification of band intensity as a ratio of FASN protein band intensity relative to the β-actin band intensity in the same blot (e.g. FASN/β-actin). For the effect of capsaicin on intracellular long chain fatty acid (<b>B</b>) and triglyceride synthesis (<b>C</b>) were determined by using the free fatty acid and triglyceride quantification kits, data are represented as percentage of the individual value in nmol/L compared to 100% of control values. (<b>D</b>) The bar chart shows the percentage of disruption of ΔΨm compared to the control (set as 100%). The change in ΔΨm was determined by staining cells with JC-1 fluorescence dye and detected by flow cytometry. Data from at least three independent experiments performed in triplicate are shown as means ± SD, n = 3, *<i>p</i><0.05.