Restoration of CD8 T-cell proliferation by CD160/CD160-ligand blockade directly correlates with the level of the <i>ex vivo</i> CD160 expression.

<p><b>(A–B)</b> Representative flow cytometric profile of 2B4, CD160 and PD-1 expression of CMV (#215 CMV HLA-A*02 NLVPMVATV) and EBV (#762 EBV HLA-B*07 RPPIFIRRL)-specific CD8 T cells detected by multimer staining (red) compared with total CD8 T cells (black/grey). <b>(C–D)</b> Representative examples of CMV (#215 CMV HLA-A*02 NLVPMVATV) and EBV (#762 EBV HLA-B*07 RPPIFIRRL)-specific CD8 T-cell proliferation in presence or not of anti-CD160 mAbs assessed by CFSE-based assay. <b>(E)</b> Impact of CD160 expression on the restoration of CD8 T-cell proliferation by CD160/CD160-ligand blockade. <b>(F–G)</b> Correlation between fold increase in proliferation capacity (y axes) upon CD160 blockage and percentage of 2B4⁺CD160⁺PD-1⁺ or 2B4⁺CD160⁺PD-1⁻ virus-specific CD8 T cells. <b>(H–J)</b> Correlation between fold increase in proliferation capacity (y axes) upon PDL-1/2 blockage and percentage of 2B4⁺CD160⁺PD-1⁺, 2B4⁺CD160⁻PD-1⁺ or 2B4⁻CD160⁻PD-1⁺ virus-specific CD8 T cells (x axes). Statistical significance (<i>P</i> values) in <b>B</b>–<b>I</b> were obtained using Spearman's rank correlations.