Comparable yields and phenotypes of BMDC and BMM generated from cathepsin B- and cathepsin L-deficient mice.

<p>BMDC from Ctsb^−/− and Ctsl^−/− mice present similar morphologies as WT BMDC. (A) Light microscopy pictures, using 40× magnification, of BMDC stained with Diff-Quik, 1: WT, 2: Ctsb^−/−, 3: Ctsl^−/−, and TEM pictures of BMDC, using a 4400× magnification, 4: WT, 5: Ctsb^−/−, 6: Ctsl^−/−. (B) No significant differences in the amount of CD11c⁺ cells generated per plate were found in Ctsb^−/− and Ctsl^−/− mice in comparison with WT mice. The results are expressed as mean ± SD of cells recovered per plate from 3 animals. (C) Similar morphologies found in BMM derived from WT and cathepsin-deficient mice (Diff-Quik staining 1: WT, 2: Ctsb^−/−, 3: Ctsl^−/−; TEM 4: WT, 5: Ctsb^−/− and 6: Ctsl^−/−). (D) No significant differences were found in the amount of F4/80⁺ cells generated per plate from WT, Ctsb^−/− and Ctsl^−/− mice.