RIP2 regulates caspases-11 expression through a ROS-JNK pathway.

<p><b>(A)</b> WT and <i>Rip2^−/−</i> BMDM were infected with 20MOI of <i>C. rodentium</i> for 18 h and supernatants collected and examined for type-I interferon using a reporter cell line (U = Units). <b>(B–C)</b> WT or <i>Rip2^−/−</i> BMDM were infected with 20MOI of <i>C. rodentium</i> and mock treated or treated with H₂O₂. Samples were collected at the indicated times and examined for total JNK or phosphorylated JNK by Western blot. <b>(D)</b> WT or <i>Rip2^−/−</i> BMDM were infected with 20MOI of <i>C. rodentium</i> and mock treated or treated with the JNK inhibitor SP600125 (JNKi). Samples were collected at the indicated times and examined for total JNK, phosphorylated JNK and caspase-11 by western blot. <b>(E)</b> Proposed signaling pathway that regulates inflammasome activation. (A) Data are combined from 9 independent experiments and displayed as the mean ± SEM. (B–D) Data are representative of 3 independent experiments. (n.s. = not significant).