Aggravated DSS-induced colitis in IL-17KO mice is associated with reduced number of CD11b⁺Ly6C⁺MHC Class II⁺ macrophages in the inflamed colon.

<p>IL-17KO mice and WT controls were treated with DSS as described in the legend for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108494#pone-0108494-g001" target="_blank">Figure 1</a>. (A) LPLs were purified from pooled (n = 2–3) distal colon sections of untreated and DSS-treated mice and subjected to flow cytometry analysis. Gating strategy of live and CD45⁺ for CD11b⁺ cells was shown in upper panels. Representative data from seven independent experiments are shown. Number denotes frequency of gated cells. (B) The frequency of cells for each subset in A is shown. Graphs represent mean ± SEM of seven independent experiments. *p<0.05. (C) Representative flow cytometry profiles of cell surface molecules implicated in M2/anti-inflammatory macrophages on each subset depicted as in A. Gray histograms represent cells stained with isotype matched control mAbs.