<i>L. mexicana</i> promastigotes activate PTPs in DCs.

<p>(<b>A</b>) In gel assay of cell extracts from DC 2.4 cells infected with <i>L. mexicana</i> promastigotes for 0.5, 1, 2, 3, 6 and 24 hr. (<b>B</b>) pNPP assay of cell lysates of DC 2.4 cells infected with <i>L. mexicana</i> promastigotes for 0.5, 1, 2 3, 6 and 24 hr. (<b>C</b>) Western blot analysis of cell lysates from <i>L. mexicana</i>-infected DC2.4 cells (0.5, 1, 2 and 3 hr) and blotted against p-ser (right panel) or p-tyr (left panel). Actin was used as loading control. In all cases, DCs were infected in a 1∶20 cell-parasite ratio. One representative experiment of three is shown.