Evaluation of IgE receptor expression on circulating eosinophils from BP patients.

<p>Peripheral granulocytes were stained with fluorescently tagged antibodies specific for human CD203c, CD49d, CD16, and FcεRI-α, FcεRIβ, CD23 or IgE for flow cytometric analysis. Eosinophils were identified by gating on the CD16⁻/CD49d⁺/CD203c⁻ population. Eosinophils from healthy controls (A), active BP patients (B), or basophils (CD16⁻/CD49d⁺/CD203c⁺) from active BP patients (C) were evaluated. The degree of specific staining is indicated by the open histogram compared to appropriate isotype control shown by the shaded histogram. Staining is representative of 10 BP patients and 11 age- and gender-matched controls.