L-DOPA triggers H3K27me3S28 phosphorylation in MSNs of hemiparkinsonian mice.

<p>A) Hemiparkinsonian mice were generated by unilateral injection of 6-OHDA into the medial forebrain bundle, eliminating the dopaminergic (DA)-innervation from the substantia nigra into the striatum. B) Acute L-DOPA administration (1 hour) increased H3S28p and H3K27me3S28p in the lesioned striatum shown by Western blotting. Mean values ±S.E.M in the bar diagrams, *** p<0.001, n = 3–6 per condition. C) Confocal micrographs of H3K27me3S28p immunostaining from the striatum of D1- or D2-receptor EGFP-expressing mice. Acute L-DOPA administration increased H3K27me3S28p which predominantly co-localized with EGFP-labeled cells of D1R-EGFP mice (t₍₄₎ = 8.20, ** p<0.01) and which segregated from EGFP-labeled cell bodies of D2R-EGFP mice (t₍₂₎ = 30.30, ** p<0.01). n = 2–3 per condition. Quantifications are means ±S.E.M. Scale bar is 30 µm.