β1 creates a leftward shift in the G-V relationship of BK channels.

<p>A) The macroscopic currents recorded from HEK 293 cells stably transected with hSlo α with 5 uM Ca²⁺ inside the pipette and in the bath. The holding potential is −60 mV, traces from 0 to 220 mV with delta 20 mV. B) G-V relations determined at 1, 5, and 10 µM Ca²⁺ concentrations. C) The macroscopic currents recorded for hSlo αβ1 at 5 µM Ca²⁺. The holding potential is −60 mV, and traces from −60 to 160 mV with delta 20 mV. D) G-V relations determined at 1, 5, and 10 µM Ca²⁺ concentrations for hSlo αβ1. Each curve in B and D represents the average of between 3 and 7 individual curves. Error bars indicate SEM. E) Plots of half-maximal activation voltage (V_1/2) vs. Ca²⁺ concentration. V1/2 were statistically different between hSlo and hSlo αβ1 for every calcium concentration tested. Error bars represent SEM, <i>n</i> = 4–7.