Morphology and differential counts of hemocytes in <i>Galerucella</i>.

<p>Morphology of hemocytes in (A) <i>G. calmariensis</i> and (B) <i>G. pusilla.</i> The white arrows indicate the cell types specified in the column headings. Both granulocytes (Gr) and prohemocytes (Pr) are shown in the first image. The orange arrowheads in the phagocyte images indicate the fluorescently-labelled bacteria inside the cells. Cell nuclei are stained with DAPI (blue). Scale bars: 20 µm. (C) Statistical table for separate ANOVAs, following the MANOVA, for six cell types between larvae of the two species (<i>G. calmariensis</i> and <i>G. pusilla</i>) and depending on parasitism by <i>A</i>. <i>parviclava</i>. F- and p-values (in brackets) are included and df_error = 56 (S: Species effect, P: Parasitism effect, S × P: Interactive effect). The column diagram shows differential hemocyte counts from laboratory-reared non-infested and infested <i>Galerucella</i> larvae (<i>G.c</i>: <i>G</i>. <i>calmariensis</i> [N_non-inf = 12, N_inf = 21], <i>G.p</i>: <i>G</i>. <i>pusilla</i> [N_non-inf = 11, N_inf = 16]). Error bars indicate standard error of the mean.