Localisation and quantification of GABA_AR alpha1 subunit immunoreactivity within the CA1 region of the hippocampus of WT and dystrophin-deficient mice, reacted and imaged under identical conditions.

<p>(A 1) shows that, in WT tissue, alpha1 subunit immunoreactivity is enriched within the dendritic layers of <i>strata oriens</i> and <i>radiatum</i> with comparatively lower levels evident in <i>strata pyramidale</i> and <i>lacunosum moleculare</i>. (A 2) shows that within <i>stratum pyramidale</i>, the majority of alpha1 subunit immunoreactive clusters colocalise with clusters immunopositive for NL2 on Kv2.1 somato-dendritic cell surfaces. (B 1) shows that within the CA1 region of mdx mice, there is an apparent increase in the intensity of alpha1 subunit immunoreactivity within <i>strata oriens</i> and <i>radiatum</i> when compared to WT. (B 2) shows that within <i>stratum pyramidale</i>, there is comparatively less colocalisation between alpha1 subunit and NL2 immunopositive clusters. (C 1) shows that within the CA1 region of mdx^βgeo mice, there is a striking decrease in the intensity of alpha1 subunit immunoreactivity throughout all layers when compared to WT. Accordingly, (C 2) shows that there is significantly less colocalisation between alpha1 subunit and NL2 immunopositive clusters when compared to WT. (D 1) quantification of the intensity of alpha1 subunit immunoreactivity within <i>stratum radiatum</i> of WT, mdx and mdx^βgeo tissue. (D 2) quantification of the density of alpha1 subunit immunoreactive clusters in <i>stratum pyramidale</i> of WT, mdx and mdx^βgeo tissue. (D 3) quantification of the density of alpha1 subunit immunoreactive clusters colocalised with clusters immunoreactive for NL2 in <i>stratum pyramidale</i> of WT, mdx and mdx^βgeo tissue. Bars represent the means and lines the SEM. *<i>P</i><0.05; ANOVA with Tukey’s posthoc test; N = 3 animals. Scale bars (A, B, C, 1) 50 µm; (A, B, C, 2) 5 µm.