Foxp3 protein detected specifically in tumor-associated macrophages.

<p>(<b>A and B</b>) The spleen, the bone-marrow (BM) and the kidney-tumor (IK tumor) have been harvested at day 14 from mice injected with Renca Ch+ L+ tumor cells intra-kidney. The same organs have been harvested from naïve non-injected mice. Representative flow cytometric analysis of Foxp3 expression are represented for T regulatory cells (TReg) (stained with anti-TCRβ, anti-CD4 and anti-CD25 antibodies), macrophages, other myeloid cells (both stained with anti-F4/80, anti-CD11b antibodies), dendritic cells (DCs) (stained with CD11c antibocy) and B cells (stained with CD19 antibody) in (<b>A</b>) naïve BALB/C mice and (<b>B</b>) Renca Ch⁺L⁺ IK tumor-bearing BALB/C mice. Black line represents FJK-16S clone for anti-Foxp3 antibody and grey line a Rat IgG2A isotype control (eBioscience, clone eBR2a). (<b>C</b>) Representative flow cytometric staining on TReg (stained with anti-TCRβ, anti-CD4 and anti-CD25 antibodies) from naïve spleen (left panel) and myeloid cells (stained with CD11b antibody) from naïve bone marrow (right panel) with FJK-16S anti-Foxp3 antibody (grey dashed line) and two different clones for rat IgG2A isotype control: clone eBR2a from eBisocience (black line) and clone R35-95 from BD Bioscience (grey line).