Luminescence from mycobacteria within macrophages.

<p>Luminescence detected from firefly (FFlux) and click beetle red (CBRlux) luciferase expressing BCG (BCG16 and BCG26) or BCG carrying the vector (pJDC89) alone within J774A.1 macrophages. Infections in macrophages were carried out with various multiplicities of infection (MOI) from 0.1 to 100 bacteria per cell for 30 min and washed to remove extracellular mycobacteria. Luciferin was added immediately after infection (day 0) and on days 2 and 7 post-infection ∼15 min before luminescence measurements. Data and error bars represent the means and standard deviations, respectively, of triplicate samples. RLU = relative light units. * indicate data points with P<0.05 for luciferase vs. pJDC89 expressing BCG at the same time point and same MOI.