The accumulation of Treg and IMC in the spleen of Apo E^−/− mice.

<p>(a), Splenocytes were stained with FITC-CD4 and PE-FoxP3 antibody, the frequencies of Treg cells at time points mentioned above were measured by flow cytometry. Representative plots are shown in (b). Data are the mean ± SEM (n = 4) of one representative experiment out of three performed. (c), Splenocytes were stained with FITC-Gr-1 and PE-CD11b antibody, the frequencies of IMC in the spleen at indicated time points were measured by flow cytometry. Representative plots are shown in (d). Data are the mean ± SEM (n = 4) of one representative experiment out of three performed. n.s = not significant, *p<0.05, **p<0.01.