Doxycycline inhibits VEGF-C/VEGFR3 signalling, macrophage infiltration and inflammatory cytokine expression in corneas with ILA.

<p>The expression of VEGF-C mRNA (A) and VEGFR3 protein (B) in the corneas were detected using real-time PCR and Western blotting, respectively. (C): Quantification of CD11b and F4/80 labelled macrophages in the corneas. (D): Representative images of CD11b and F4/80 labelled macrophages in the corneas of different groups. The expression of IL-1β (E) and TNF-α (F) mRNA in the corneas was determined using real-time PCR. **<i>p</i><0.01, *** <i>p</i><0.001. The scale bars represent 25 µm.