Activated γδ T cells show increased ability to bind adenosine.

<p>A–B) 1×10⁵ γδ T cells from IRBP_1-20-immunized (A) or naïve (B) B6 mice were incubated with 0 to 12,000 nM H³-labeled adenosine for 1 h, then the cells were harvested and cell-bound H³-adenosine counted by liquid scintillation. C). A2AR is the major AR on γδ T cells from immunized B6 mice. In 96-well plate γδ T cells (1×10⁵/well) from IRBP_1-20-immunized mice were incubated with 100 nM radiolabeled adenosine alone or after 1 h preincubation with 10 µM NECA or 100 nM A2AR antagonist (SCH 58261) and/or A2BR antagonist (MRS 1754). ** p<0.01.