Effect of ZFC3H1 on <i>IL-8</i> gene transcription.

<p>(A) Structure of the human <i>IL-8</i> promoter. AP-1, C/EBP, and NF-κB sites are displayed. In the mutant constructs, underlined nucleotides were replaced. (B) IL-8(−238–+29)-pGL4.11 and the internal control vector, pGL4.74, were transfected into LU99 cells, and their luciferase activities were measured 6 h after the TNFα (20 ng/ml) stimulation. Firefly luciferase activity was divided by Renilla luciferase activity. Three independent experiments. (C) IL-8-pGL4.11 with or without mutations was co-transfected with pGL4.74 into LU99 cells. LU99 cells were treated with control or ZFC3H1 siRNA prior to the experiments. Cells were stimulated with TNFα (5 ng/ml) for 6 h. Firefly luciferase activity was divided by Renilla luciferase activity. pGL4.11 is a blank vector, and this data was set as 1. Three independent experiments. *P<0.05. NS, not significant.