Force-Ca²⁺ relationship obtained from GAS (A) and EDL (B) skinned muscle fibers.

<p>Force was generated in response to incubation with incremental increase of [Ca²⁺]. Note the slight leftward shift of the force–Ca²⁺ relationship in GAS Tg(<i>TPM3</i>)^Met9Arg fibers (○, n = 50) as compared to GAS WT fibers (•, n = 49) (left panel Fig. A), resulting in a higher pCa₅₀ values and <i>n</i>_H value (right panel Fig. A) in Tg(<i>TPM3</i>)^Met9Arg fibers (ΔpCa₅₀ = 0.07; middle panel Fig. A). On the contrary, both curves were overlapped in EDL muscle fibers (n = for 50 WT and n = 48 for Tg(<i>TPM3</i>)^Met9Arg, left panel Fig. B), resulting in a similar pCa₅₀ value (middle panel Fig. B) and <i>n</i>_H value (right panel Fig. B). Values are presented as the mean ± SEM. Significantly different between groups *<i>P</i><0.05.