Light production of the media control wells and media reference wells in the AI-2 reporter assay.

  • Beth L. Zimmer (636592)
  • Amanda L. May (415085)
  • Chinmayee D. Bhedi (636593)
  • Stephen P. Dearth (636594)
  • Carson W. Prevatte (636595)
  • Zoe Pratte (636596)
  • Shawn R. Campagna (211553)
  • Laurie L. Richardson (636597)
Publication date
September 2014

Abstract

<p>The medium control curves indicate that the three growth media used in this study did not stimulate light production prior to self-induction by the <i>Vibrio harveyi</i> BB170 reporter strain. The luminescence of the medium reference wells, which contained sterile growth media, remained minimal over the course of the AI-2 reporter assay, although some minor increases in light were measured in these wells at the end of the assay due to light contamination from adjacent wells. Arrow indicates time of self-induction by the reporter strain, after which the luminescence of the reporter strain increases rapidly.</p

Extracted data

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