Fluvastatin induces cell death in MDA-MB-231 breast cancer cells: Effect of mevalonate.

<p>A, MDA-MB-231 cells were treated with various concentrations of fluvastatin (0–20 µM) for 48 h and B shows cells treated for different time points with 10 µM fluvastatin. At the end of the treatments, cell viability was measured by trypan blue exclusion method. C, MCF-10A cells were treated with fluvastatin (0–20 µM) for a period of 24 and 48 h and cell death was measured as described earlier. D, MDA-MB-231 cells were treated with fluvatstatin (10 µM) in presence or absence of mevalonate (25 µM) for a period of 48 h and cell viability was measured by trypan blue exclusion assay. Data represented is Mean±SD from at least three independent experiments. *, significantly different compared to untreated conditions; #, significantly different compared to mevalonate and fluva+mev condition. Statistical significance was tested at P<0.05 level by two-tailed, unpaired, Student's t-test.