Loss-of-function genetic screen identifies positive regulators of antiviral responses.

<p><b>(A)</b> Protocol schematic of the primary genetic screen. <b>(B)</b> Ranked range normalization plot of the 827 shRNA of interest and controls. Range normalized HSV-GFP infection efficiency for each individual shRNA-transduced well, expressed as percentage within range 0 to 1, is ranked from low to high and displayed. Green bars indicate normalized irrelevant shRNA controls and red bars indicate normalized positive controls. Grey bars represent normalized shRNA targeting individual genes within T1D susceptibility loci. Location of shRNAs with a Z score equal or greater than 3 is indicated by the area shaded in blue. <b>(C)</b> Representative images are shown for two high-scoring shRNAs that target <i>Tagap</i> and <i>Il27</i> respectively, and one irrelevant shRNA that targets lacZ. Hoechst nucleic acid stain indicates the total cells per microscopic field and GFP indicates the virally infected cells.