Comparison of AMPA receptor densities.

<p>A) A raw electron micrograph image of SDS-FRL replica immunolabeled with pan-AMPA antibody (left), and an analyzed image (right). A representative replica from a wild type mouse treated with saline (WT-Sal) is shown. The blue area indicates intra-membrane particle clusters and the black dots indicate immunogold particles. B) Densities of pan-AMPA, GluR1, and GluR2 in WT-Sal mice and β2m mice treated with saline (β2m^−/−-Sal). The densities normalized to WT in each receptor were: pan-AMPA: β2m^−/−-Sal, 110.0±8.5%; GluR1: β2m^−/−-Sal, 92.9±7.8%; GluR2: β2m^−/−-Sal, 95.4±1.5%, n = 6 [for each receptor 6 replicas from three animals, 30 synapses per replica]. C) Comparison of pan-AMPA, GluR1 and GluR2 densities between WT-Sal mice and wild type mice treated with cocaine (WT-Coc). The densities normalized to saline group in each receptor were: pan-AMPA: WT-Coc, 119.4±8.3%; GluR1: WT–Coc, 135.3±8.9%; GluR2: WT–Coc, 105.6±9.6%, n = 6 [for each receptor six replicas from three animals, 30 synapses per replica]. D) Comparison of pan-AMPA, GluR1 and GluR2 densities between β2m^−/−-Sal mice and β2m^−/− mice treated with cocaine (β2m^−/−-Coc). The densities normalized to saline group in each receptor were: pan-AMPA: β2m^−/−-Coc, 130.9±10.0%; GluR1: β2m^−/−-Coc, 117.5±5.8%; GluR2: β2m^−/−-Coc, 119.7±6.2%, n = 6 [for each receptor six replicas from three animals, 30 synapses per replica]. E) Representative electron micrographs of the replicas from WT-Sal and WT-Coc mice. The blue area indicates intra-membrane particle clusters and the black dots indicate immunogold particles. F) Representative electron micrographs of the replicas from β2m^−/−-Sal and β2m^−/−-Coc mice. The red area indicates intra-membrane particle clusters and the black dots indicate immunogold particles. Scale bars represent 200 nm. *p<0.05.