Changes in muscular signalling after cisplatin treatment.

<p>Gene expression of A) Atrogin-1 and B) MuRF-1 was determined in muscles from cisplatin-treated (CIS) or saline-treated mice (Control), randomized to exercise training during treatment (Ex). Ratio between phosphorylated and total C) Akt (Ser473) and D) mTOR (Ser2448) was measured by Western blotting. Total-Akt and total-mTOR protein abundance did not differ significantly between groups (data not shown). For pAkt/Akt, a significant effect of exercise (P<0.001) and cisplatin treatment (P<0.0001) was observed in the 2-way ANOVA, while for p-mTOR/mTOR, a significant effect of cisplatin treatment (P<0.001) was observed, (No Ex = 8, Ex = 9). E) Muscle glycogen content, and F) muscular ion content. Results are presented as means ± SEM (n = 8). Statistical analysis was performed by 2-way ANOVA with Bonferroni's post hoc test. *P<0.05, **P<0.01, ****P<0.0001 indicates statistical significance compared to non-exercising control or as indicated by the lines.