MP wound infiltration was localized to the deep skin layer.

<p>(<b>a</b>) Representative picture of skin wound excision. Pictures represent (<b>b</b>) front and (<b>c</b>) transversal two-photon laser scanning microscopy (TPLSM) 3D reconstruction of the wound site of a C57Bl6 mouse, 3 h after injury. The second harmonic generation (SHG) signal is in blue, and autofluorescent hairs in green. (<b>d</b>) Representative dot plot showing GFP and ECFP expression on distinct blood myeloid subsets from MacBlue×CX3CR1^gfp/+ mice. (<b>e</b>) Front and (<b>f</b>) transversal TPLSM 3D reconstruction of the wound site in MacBlue×CX3CR1^gfp/+ mouse, 4 h post-wounding. The SHG signal is in blue, the ECFP signal in cyan, and the GFP signal in green. Legends represent <b>1</b>: Skull; <b>2</b>: Deep skin layers; <b>3</b>: Wound edge; <b>4</b>: Skin. Inf Mo: Inflammatory monocytes; Np: neutrophils; Resident Mo: resident monocytes.