Androgenic response of PC-3 cells over-expressing AR and Skp2.

<p>(A) Protein expression of Skp2 was assayed by Western blotting in PC-3 cells re-expressing wild type AR (PC-3^AR) cells and overexpressing either control vector or Skp2 in the absence or presence of 10 nM R1881 for 96 h. Effect of androgen on cell proliferation (B) or cell cycle progression (C) of these clones treated with increasing concentration of R1881 for 96 h was determined by 96-well proliferation assay and PI-staining flow cytometry analysis, respectively. Asterisks * and ** denote a significant difference <i>p</i><0.05 and <i>p</i><0.01, respectively, between the treatment and the control cells.