Most B cells in BLT mouse tissues exhibit an immature phenotype.

<p>(<b>A–B</b>) Flow cytometry plots and bar graph for CD27 and IgD expression on B cells reveal an absence of unswitched/switched memory B cells in BLT mouse BM, spleen, LN, liver and lungs (n = 5 for all tissues). (<b>C–D</b>) Flow cytometry plots and bar graph for CD38 and IgD expression on CD19⁺ cells reveal an absence of switched memory B cell populations in BLT mouse tissues (n = 5 for all tissues). * indicates a p value less than 0.05. ** indicates a p value less than 0.01. *** indicates a p value less than 0.001. Comprehensive statistical analyses are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0108663#pone-0108663-t002" target="_blank">Table 2</a>.