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Pre-tRNA cleavage assay of the recombinant proteins r67 and r104.

  • Chieko Sugita (637607)
  • Yoshihiro Komura (637608)
  • Korechika Tanaka (637609)
  • Kazuki Kometani (637610)
  • Hiroyuki Satoh (637611)
  • Mamoru Sugita (637612)
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Publication date
October 2014
Icon DOI
10.1371/journal.pone.0108962.g006

Abstract

<p>Reactions containing 500 nM of chloroplast 5′-<sup>32</sup>P-pre-tRNA<sup>Arg</sup> (ACG) (upper), tRNA<sup>Arg</sup> (CCG) (middle), or tRNA<sup>Arg</sup> (UCU) (lower) and 500 nM of recombinant protein were quenched at 1, 5, 15, and 30 min points, resolved on 8% denaturing PAGE, and analyzed with a phosphoimager.</p

Extracted data

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