Alterations in serum insulin and leptin levels.

<p>A, B. Circulating insulin concentration was measured in blood samples from all genotypes at 4 months of age by ELISA. Serum insulin levels were significantly higher in BACHD and BACHD-SF1 females compared WT and SF1 controls while in males, only the BACHD group had significantly enhanced insulin concentration compared to SF1 controls. C, D. Serum leptin concentration as measured by ELISA was significantly higher in BACHD and BACHD-SF1 mice compared to WT and SF1 controls in both males and females. Data presented as mean ± SEM (A–B, * p<0.05, Kruskal-Wallis non-parametric ANOVA with Dunn’s multiple comparisons post-hoc test, C–D, * p<0.05, one-way ANOVA with Tukey multiple comparisons post-hoc test), n = 11–28/genotype/sex in each group. Detailed statistical analyses are outlined in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107691#pone-0107691-g003" target="_blank">Figure 3</a> of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0107691#pone.0107691.s002" target="_blank">Statistical Results S1</a>.