Immunostaining of primary Müller cells isolated from the conditional <i>β-catenin</i> KO mice.

<p>(<b>A–E</b>) Primary Müller cells from the conditional <i>β-catenin</i> KO mice were co-immunostained with an antibody against GS (green) (<b>A</b>) and an antibody for β-catenin (red) (<b>B</b>). The nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI, blue <b>C</b>). Immunostaining of GS (green) and β-catenin (red) in primary Müller cells from the β-catenin KO mice merged with nuclei stained with DAPI (blue) (<b>D</b>). Negative IgG is used as control (<b>E</b>). Arrows indicate Müller cells lacking β-catenin expression. Scale bar: 50 µm. Real-time PCR analysis of β-catenin mRNA levels in the primary Müller cells (<b>F</b>). Values are mean±SEM; n = 3; *p<0.05.