The phosphorylation of MAPK Cmk1 in the <i>coira1</i> mutant.

<p>(A) The total protein isolated from mycelia of each strain. WT; the wild-type, DL1; the <i>coira1</i> mutant, DRS2; the <i>coras2</i> mutant, DARS1, WT transformed with a dominant active form <i>CoRAS1</i>, DARS2; WT transformed with a dominant active form <i>CoRAS2</i>, iDNRS1; the <i>coira1</i> mutant transformed with a dominant negative form <i>CoRAS1</i>, iDNRS2; the <i>coira1</i> mutant transformed with a dominant negative form <i>CoRAS2,</i> DCK1; the <i>cmk1</i> mutant The anti-phospho p44/42 MAPK antibody detected a 41-KD Cmk1 and 47-KD Maf1. The anti-actin antibody detected a 42-KD actin. (B) Relative activity of MAPK Cmk1 phosphorylation of each mutant was calculated by comparison of signal intensity with that of the wild-type, normalized by actin signal. The quantitative analysis of phosphorylated Cmk1 was performed by four replicated experiments. Asterisk represents significant differences between the wild type and each mutant. (Student’s <i>t</i> test: *indicate <i>P</i><0.05).