IL-32γ decreases caspase-3 activity in neutrophils.

<p>Neutrophils were incubated with IL-32γ (500 ng.ml-1) or its vehicle (CONT) for 48 hrs. Cells with activated caspase-3 were then labelled with the caspase-3 inhibitor DEVD-FMK-FITC before being analyzed by FACS. The results are means ± s.e.m. of six different donors. Statistics: paired Student <i>t</i> test (^*** p<0.001) (<b>A</b>). Cells undergoing apoptosis were also stained with a specific antibody against the caspase-3 active form (cleaved caspase-3) before FACS analysis. The results are means ± s.e.m. of three different donors. Statistics: paired Student <i>t</i> test (^*** p<0.001) (<b>B</b>).