Comparison of the MDM2-binding modes between MIP and other peptides.

<p>(<b>A</b>) superpositions of MIP-MDM2 fusion (PDB ID = 2RUH), DI:MDM2 (3G03), PMI:MDM2 (3EQS), and p53 peptide:MDM2 (1YCR) in ribbons, viewed as in Fig. 2A, right. Each component is color coded: MIP (brown)-MDM2 (orange), DI (blue):MDM2 (cyan), PMI (pink):MDM2 (purple), and the p53 peptide (green):MDM2 (light green). (<b>B</b>) MIP, DI, PMI, and p53 peptide are presented as two opposite views. W4, Y6, and M11 of MIP cover the hydrophobic patches on the surface of MDM2, as shown in Fig. 2C, left. These residues and the Phe-Trp-Leu triad, and the corresponding residues in DI, PMI, and the p53 peptide are displayed as sticks. (<b>C</b>) A-zoomed-in view of the hydrophobic contacts formed around W4 and M11 of MIP. Fig. 3A, Fig. 3B (left), and Fig. 3C are views from the same direction as in Fig. 2A (right), Fig. 2B (left), and Fig. 2C (left).