Phylogenetic analysis of Japanese encephalitis virus.

<p>The phylogenetic tree shows the genetic relationship of genotype I (A) and genotype III (B) of Japanese encephalitis virus isolates. The tree was constructed based on the nucleic acid sequences of complete envelope (E) genes of the JEV strains. The sequences obtained in this study are indicated in <b>boldface</b>. A sequence obtained from a human case is indicated with a triangle (▴). Viruses were identified by using the nomenclature of virus/country/strain/source/year of isolation/GenBank accession number. Numbers in parentheses indicate the number of isolates that showed 100% nucleotide homology. Virus isolates with the same sequences were collected at the same time from the same locations in this study. CH = Changhua County; HL = Hualien County; KH = Kaohsiung City; TC = Taichung City; TN = Tainan City; TP = New Taipei City; TPC = Taipei City; TY = Taoyuan County; YL = Yilan County; M = mosquito pool; P = pig serum; H = human sample. Analysis was performed using MEGA 5 software and the maximum likelihood method based on the general time-reversible model. The reliability of the analysis was calculated using 1,000 bootstrap replications. Bootstrap support values>75 are shown. The scale bar indicates nucleotide substitutions per site.