Properties of CSCs obtained from docetaxel-resistant human lung adenocarcinoma cells (SPC-A1/DTX and H1299/DTX).

<p>(A₁, A₂, A₃) Percentage of CD133⁺/CD326⁺ CSCs as analyzed by Flow cytometry at the indicated time after cultivating CD133⁺/CD326⁺ CSCs (acquired by sorting SPC-A1/DTX and H1299/DTX cells, respectively) under differentiation conditions. (B) Mammosphere forming ability of the indicated cells (1000 cells) after 7 days under CSC-cultivating conditions. (C) qRT-PCR detection of relative mRNA levels of the CSC-related markers in the indicated cells. Data was calculated with the 2^−△△Ct method using GAPDH RNA as the reference gene. The expression level was measured relative to the fold change of the parental cells groups that were defined as 1.0. (D₁, D₂) The protein levels of the CSC-related markers in the indicated cells. GAPDH was used as an internal control. (E) Tumor incidence in nude mice that were subcutaneously injected with the indicated cells. Data were presented as mean ± SD of at least three independent experiments. *<i>p</i><0.05, **<i>p</i><0.01.