BORIS promoter methylation and expression of <i>hTERT</i>, stem cell and CSC markers genes in BORIS-positive cells.

<p>(A) Methylation analysis of 10 CpG islands within the <i>BORIS</i> promoter region (B promoter). The representative graphic shows the percentage of methylation of each CpG island for the isolated BORIS-positive (white), -negative (grey) and non-sorted (black) NCCIT cells. (B) Expression analysis of the isolated BORIS-positive (white) and BORIS-negative (grey) fractions. The indicated genes were analyzed by qRT-PCR. The results were normalized to <i>GAPDH</i> and related to non-sorted NCCIT cells. Graphic shown one representative experiment out of 3 independent experiments (the trend was similar in all independent experiments). Asterisks indicate statistically significant difference (p<0.05) between BORIS-positive and the non-sorted cells.