Effects of rno-miR-376a on GRP78 protein in granulosa cells.

<p>Primary rat granulosa cells were prepared, and the indicated reagents were added to the medium after 24 h of culture. The cells were incubated with FSH (30 ng/mL) and estradiol (10 nM) for 48 h. Negative control (A), precursor (B) or inhibitor (C) was transfected into the cells, and 30 ng/mL hCG was added 12 h later. Cells were harvested 24 h after the addition of hCG, and GRP78 protein levels were quantified using western blot analysis. (D) Levels of GRP78 protein were quantified by densitometric scanning. The expression of GRP78 protein in the control (hCG 0 h) was set at 1. Values represent the mean ±SE of three independent experiments. *, significantly different from the control value, <i>P</i><0.05.