Effects of 53 novel chemicals on intracellular triglyceride (TG) synthesis during 3T3-L1 adipocyte differentiation.

<p>3T3-L1 preadipocytes were induced to differentiate with induction medium containing MDI (M, IBMX (0.5 mM); D, dexamethasone (0.5 µM); I, insulin (5 µg/ml)), insulin (5 µg/ml), and FBS (10%) in the presence or absence of 10 µM of the test compounds for 8 days. On day 8, the cellular TG contents were quantified by AdipoRed assay. Values are mean ± SE of data from three independent experiments with three replicates. *P or ^#P<0.05 and **P or ^##P<0.01 vs. control (no chemical).