Self renewal ability of HSPC is not affected by Caspase-3 and/or p21^Cip1/Waf1 deletion.

<p>(A) Scheme of the serial transplantation assay that was performed to analyze the ability of the different depleted HSCs to sustain the proliferative stress of repetitive transplantations rounds. (B) Analysis of peripheral blood counts 17 weeks after 2^nd round of bone marrow transplantation showed the same reduced white blood cell contribution of the Caspase-3^-/- as the one observed in the 1^st round of transplantation. (C) FACS analysis of peripheral blood cells 17 weeks after 2^nd round of bone marrow transplantation showed that simultaneous depletion of p21^Cip1/Waf1-/- rescues the effect of Caspase-3^-/- depletion in the different cell types. (D) No differences were found in the percentage of LKS cells in bone marrow 20 weeks after 2^nd round of bone marrow transplantation. Values are mean ± SEM; n≥3; *p≤0.05.