Bioluminescent Probe for Hydrogen Peroxide Imaging in Vitro and in Vivo

Reactive oxygen species (ROS) often have significant roles in mediating redox modifications and other essential physiological processes, such as biological process regulation and signal transduction. Considering that H₂O₂ is a substantial member of ROS, detection and quantitation of H₂O₂ undertakes important but urgent responsibility. In this report, a bioluminescent probe for detecting H₂O₂ was well designed, synthesized, and evaluated. This probe was designed into three parts: a H₂O₂-sensitive aryl boronic acid, a bioluminescent aminoluciferin moiety, and a self-immolative linker. After extensive evaluation, this probe can selectively and sensitively react with H₂O₂ to release aminoluciferin. It should be pointed out that this probe is a potential bioluminescent sensor for H₂O₂ since it can provide a promising toolkit for real-time detection of the H₂O₂ level in vitro, in cellulo, and in vivo