Karyotyping and chromosome localization of AS in <i>Hylobates lar,</i> Conlar and Larcon.

<p><b>A</b> and <b>B</b>, <i>Hylobates lar entelloides</i> (male). <b>C</b> and <b>D</b>, Thai hybrid offspring between <i>Hylobates lar</i> (HLA) and <i>Nomascus leucogenys</i> (NLE) (female) (NLE/HLA, Conlar). <b>E</b> and <b>F</b>, Japanese hybrid offspring (female) (HLA/NLE, Larcon). <b>A), C)</b>, and <b>E)</b> DAPI-band (G-like band) reversed from the light fluorescence band of DAPI. <b>B), D)</b>, and <b>F)</b> Localization of AS (red signal). Numbers in orange are the chromosome number of the genus <i>Hylobates</i>. Chromosomes are classified with standard karyotypes as described previously (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109151#pone.0109151-Stanyon1" target="_blank">[11]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109151#pone.0109151-Wijayanto1" target="_blank">[41]</a> for <i>Hylobates</i>; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109151#pone.0109151-Couturier1" target="_blank">[28]</a> for <i>Nomascus</i>). Numbers and letters with orange and green underlines show specific karyotype for the species HLA and NLE, respectively. Numbers with red underlines indicate chromosomes with interstitial band blocks in an arm. White arrowheads indicate the location of interstitial AS blocks.