Nucleolin protein down-regulation is involved in ADP-induced inhibition of cell proliferation.

<p>(A) Endothelial cell proliferation in response to ADP. HUVEC and HAEC were treated with the indicated concentrations of ADP for 72 h. Medium was replaced with fresh medium containing ADP every day. Cell number was measured with CCK-8 assay. * <i>P</i><0.05 compared with the control groups. (B) The effect of ADP and UDP on HUVEC proliferation. HUVEC were treated with 100 µM ADP or UDP for the indicated time periods. Medium was replaced with fresh medium containing ADP or UDP every day. Cell number was measured with CCK-8 assay. * <i>P</i><0.05 compared with the control groups. (C) Endothelial cell proliferation in response to UDP. * <i>P</i><0.05 compared with the control groups. (D) MRS2211 (MRS) did not reverse ADP-induced inhibition of cell proliferation. HUVEC, pre-treated with the indicated concentrations of MRS2211 for 30 min, were re-treated with ADP for 72 h. Medium was replaced with fresh medium containing MRS2211 and ADP every day. Cell number was measured with CCK-8 assay. * <i>P</i><0.05 compared with the control groups. (E) MRS2211 (MRS) induced inhibition of cell proliferation. HUVEC were treated with the indicated concentrations of MRS2211 for 72 h. Medium was replaced with fresh medium containing MRS2211 every day. Cell number was measured with CCK-8 assay. * <i>P</i><0.05 compared with the control group. (F) 2-MeSADP did not induce cell proliferation inhibition. HUVEC were treated with the indicated concentrations of 2-MeSADP for 72 h. Medium was replaced with fresh medium with 2-MeSADP every day. Cell numbers were measured by CCK-8 assay. (G) Nucelolin-over-expression partly reversed ADP-induced inhibition of cell proliferation. Nucleolin-over-expressed HUVEC were treated with the indicated concentrations of nucleolin for 72 h. Medium was replaced with fresh medium containing ADP every day. Mock-treansfected HUVEC were used as control. Cell number was measured with CCK-8 assay. * <i>P</i><0.05 compared with the control groups.