GLUT4 content and subcellular distribution in adipose tissue depots of 6 week-old Alms1^GT/GT mice before and after insulin stimulation.

<p>(a) Representative western blot of total GLUT4 and (b) quantification (n = 6) normalized for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) content, in subcutaneous (SAT) and visceral (VAT) adipose tissues of wt (black bars) and <i>Alms1^GT/GT</i> (white bars). (c) Representative western blot of GLUT4 distribution in the subcellular compartments (PM = plasma membrane; HD = high density microsome; LD = low density microsome) in basal conditions (bas) and after insulin stimulation (ins) in SAT pooled from 3 wt and <i>Alms1^GT/GT</i> mice. (d) The plot represents the fold-increase (insulin/basal) in GLUT4 signal after insulin stimulation in every subcellular fraction from SAT of wt (black bars) and <i>Alms1^GT/GT</i> (white bars) mice as mean values ±SEM of 3 western blot quantification. *p<0.05 wt <i>vs</i>. <i>Alms1^GT/GT</i>.